Program
MONDAY January 7
12:30 Registration / Light lunch (A11/205)
13:50 Opening
14:00 Lecture 1 Robert Konrat: IDPs in general and how NMR can provide information
15:30 Tea & coffee (A11/205)
16:00 Lecture 2 Robert Konrat: How to derive structural information about transiently formed
17:30 Welcome mixer (A35 Atrium)
TUESDAY January 8
9:00 Lecture 3 Isabelle Landrieu: Liquid state NMR spectroscopy of (disordered) proteins compact states in IDPs
10:30 Tea & coffee (A11/205)
11:00 Lecture 4 Dmitri Svergun: Small-angle scattering on macromolecular solutions: basics
12:30 Lunch (A35 Atrium)
14:00 Lecture 5 Dmitri Svergun: Small-angle scattering to study disorder and aggregation
15:30 Tea & coffee (A11/205)
16:00 Lab tour: X-ray Diffraction and Bio-SAXS core facility, Josef Dadok National NMR Centre
WEDNESDAY January 9
9:00 Lecture 6 Rostislav Škrabana: Protein disorder studied by a synergy of experiment and simulation: X-ray crystallography and biophysics
10:30 Tea & coffee (A11/205)
11:00 Lecture 7 Rostislav Škrabana: Protein disorder studied by a synergy of experiment and simulation: Molecular dynamics
12:30 Lunch (A35 Atrium)
14:00 Lecture 8 Michaela Wimmerová: Biomolecular interactions, calorimetry (SPR, DSF, MST, ITC, DSC)
15:30 Tea & coffee (A11/205)
16:00 Lab tour: Biomolecular Interactions and Crystallization core facility
THURSDAY January 10
9:00 Lecture 9 Markus Hartl: A primer on structural proteomics: MS-basics, cross-linking, HDX, ion-mobility
10:30 Tea & coffee (A11/205)
11:00 Lab tour: Cryo-Electron Microscopy and Tomography and Nanobiotechnology core facilities
12:30 Lunch (A35 Atrium)
14:00 Lecture 11 Edward Lemke: Tools to decode molecular plasticity in the dark proteome
15:30 Tea & coffee (A11/205)
16:00 Lab tour: Proteomics and Cellular Imaging core facilities
FRIDAY January 11
9:00 Lecture 12 Sonia Longhi: General methods for the assessment of disorder: PAGE, limited proteolysis, analytical size exclusion chromatography, CD, thermal shift assay, intrinsic fluorescence
10:30 Tea & coffee (A11/205)
11:00 Lecture 13 Sonia Longhi: Advanced methods of disorder investigation: vibrational spectroscopy of cyanylated cysteine, Trp-Cys quenching, site-directed spin-label EPR spectroscopy
12:30 Lunch (A35 Atrium)
